rabbit anti vitronectin nbp2 20866 Search Results


rabbit anti vitronectin nbp2 20866  (Novus Biologicals)
90
Novus Biologicals rabbit anti vitronectin nbp2 20866
Rabbit Anti Vitronectin Nbp2 20866, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti human b actin  (R&D Systems)
92
R&D Systems mouse anti human b actin
Mouse Anti Human B Actin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-human β-actin ac-15  (R&D Systems)
90
R&D Systems mouse anti-human β-actin ac-15
Mouse Anti Human β Actin Ac 15, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat anti-mouse cd11b m1/70  (Becton Dickinson)
90
Becton Dickinson rat anti-mouse cd11b m1/70
Rat Anti Mouse Cd11b M1/70, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-active tgf-β1  (Promega)
90
Promega rabbit anti-active tgf-β1
A–D C7-hypomorphic mice were treated with losartan for 7 weeks, and the forepaws of age-matched untreated, losartan-treated, and wild-type mice were subjected to immunofluorescence staining with antibodies to active thrombospondin 1 (Tsp1, green) (A), active <t>Tgf-β1</t> (green) (B), TGF-β receptor II (Tgfbr2, red) (C), and phospho-Smad2 and 3 (P-Smad2/3, red) (D). The nuclei were counterstained with DAPI (blue). Images were acquired with a 20× objective (scale bar = 100 μm) (A), with a 4× objective (scale bar = 200 μm) (B, D), and with a 40× objective (scale bar = 50 μm) (C). The bar graphs on the right show quantification of the stainings in the left panel of (B) and (D). Paired values were normalized to the staining intensity of untreated C7-hypomorphic paws, which were set to 100%. Values represent mean ± S.E.M., n = 6, paired Student’s t -test, *** P -value wild-type vs. C7-hypomorph receiving no treatment = 0.0004; *** P -value losartan treatment vs. no treatment = 0.0005 (B). Values represent mean ± S.E.M., n ≥ 7, unpaired t -test with Welch’s correction used, *** P -value wild-type vs. C7-hypomorph < 0.0001; *** P -value C7-hypomorph + losartan vs. C7-hypomorph = 0.0002; ** P -value wild-type vs. C7-hypomorph + losartan = 0.0016 (D).
Rabbit Anti Active Tgf β1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti human collagen i r1038x  (OriGene)
91
OriGene rabbit anti human collagen i r1038x
A–D C7-hypomorphic mice were treated with losartan for 7 weeks, and the forepaws of age-matched untreated, losartan-treated, and wild-type mice were subjected to immunofluorescence staining with antibodies to active thrombospondin 1 (Tsp1, green) (A), active <t>Tgf-β1</t> (green) (B), TGF-β receptor II (Tgfbr2, red) (C), and phospho-Smad2 and 3 (P-Smad2/3, red) (D). The nuclei were counterstained with DAPI (blue). Images were acquired with a 20× objective (scale bar = 100 μm) (A), with a 4× objective (scale bar = 200 μm) (B, D), and with a 40× objective (scale bar = 50 μm) (C). The bar graphs on the right show quantification of the stainings in the left panel of (B) and (D). Paired values were normalized to the staining intensity of untreated C7-hypomorphic paws, which were set to 100%. Values represent mean ± S.E.M., n = 6, paired Student’s t -test, *** P -value wild-type vs. C7-hypomorph receiving no treatment = 0.0004; *** P -value losartan treatment vs. no treatment = 0.0005 (B). Values represent mean ± S.E.M., n ≥ 7, unpaired t -test with Welch’s correction used, *** P -value wild-type vs. C7-hypomorph < 0.0001; *** P -value C7-hypomorph + losartan vs. C7-hypomorph = 0.0002; ** P -value wild-type vs. C7-hypomorph + losartan = 0.0016 (D).
Rabbit Anti Human Collagen I R1038x, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit antiserpinf2 orb101685  (Biorbyt)
85
Biorbyt rabbit antiserpinf2 orb101685
A–D C7-hypomorphic mice were treated with losartan for 7 weeks, and the forepaws of age-matched untreated, losartan-treated, and wild-type mice were subjected to immunofluorescence staining with antibodies to active thrombospondin 1 (Tsp1, green) (A), active <t>Tgf-β1</t> (green) (B), TGF-β receptor II (Tgfbr2, red) (C), and phospho-Smad2 and 3 (P-Smad2/3, red) (D). The nuclei were counterstained with DAPI (blue). Images were acquired with a 20× objective (scale bar = 100 μm) (A), with a 4× objective (scale bar = 200 μm) (B, D), and with a 40× objective (scale bar = 50 μm) (C). The bar graphs on the right show quantification of the stainings in the left panel of (B) and (D). Paired values were normalized to the staining intensity of untreated C7-hypomorphic paws, which were set to 100%. Values represent mean ± S.E.M., n = 6, paired Student’s t -test, *** P -value wild-type vs. C7-hypomorph receiving no treatment = 0.0004; *** P -value losartan treatment vs. no treatment = 0.0005 (B). Values represent mean ± S.E.M., n ≥ 7, unpaired t -test with Welch’s correction used, *** P -value wild-type vs. C7-hypomorph < 0.0001; *** P -value C7-hypomorph + losartan vs. C7-hypomorph = 0.0002; ** P -value wild-type vs. C7-hypomorph + losartan = 0.0016 (D).
Rabbit Antiserpinf2 Orb101685, supplied by Biorbyt, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hrp conjugated goat anti rabbit igg  (Jackson Immuno)
96
Jackson Immuno hrp conjugated goat anti rabbit igg
A–D C7-hypomorphic mice were treated with losartan for 7 weeks, and the forepaws of age-matched untreated, losartan-treated, and wild-type mice were subjected to immunofluorescence staining with antibodies to active thrombospondin 1 (Tsp1, green) (A), active <t>Tgf-β1</t> (green) (B), TGF-β receptor II (Tgfbr2, red) (C), and phospho-Smad2 and 3 (P-Smad2/3, red) (D). The nuclei were counterstained with DAPI (blue). Images were acquired with a 20× objective (scale bar = 100 μm) (A), with a 4× objective (scale bar = 200 μm) (B, D), and with a 40× objective (scale bar = 50 μm) (C). The bar graphs on the right show quantification of the stainings in the left panel of (B) and (D). Paired values were normalized to the staining intensity of untreated C7-hypomorphic paws, which were set to 100%. Values represent mean ± S.E.M., n = 6, paired Student’s t -test, *** P -value wild-type vs. C7-hypomorph receiving no treatment = 0.0004; *** P -value losartan treatment vs. no treatment = 0.0005 (B). Values represent mean ± S.E.M., n ≥ 7, unpaired t -test with Welch’s correction used, *** P -value wild-type vs. C7-hypomorph < 0.0001; *** P -value C7-hypomorph + losartan vs. C7-hypomorph = 0.0002; ** P -value wild-type vs. C7-hypomorph + losartan = 0.0016 (D).
Hrp Conjugated Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hrp conjugated goat antimouse igg  (Jackson Immuno)
96
Jackson Immuno hrp conjugated goat antimouse igg
A–D C7-hypomorphic mice were treated with losartan for 7 weeks, and the forepaws of age-matched untreated, losartan-treated, and wild-type mice were subjected to immunofluorescence staining with antibodies to active thrombospondin 1 (Tsp1, green) (A), active <t>Tgf-β1</t> (green) (B), TGF-β receptor II (Tgfbr2, red) (C), and phospho-Smad2 and 3 (P-Smad2/3, red) (D). The nuclei were counterstained with DAPI (blue). Images were acquired with a 20× objective (scale bar = 100 μm) (A), with a 4× objective (scale bar = 200 μm) (B, D), and with a 40× objective (scale bar = 50 μm) (C). The bar graphs on the right show quantification of the stainings in the left panel of (B) and (D). Paired values were normalized to the staining intensity of untreated C7-hypomorphic paws, which were set to 100%. Values represent mean ± S.E.M., n = 6, paired Student’s t -test, *** P -value wild-type vs. C7-hypomorph receiving no treatment = 0.0004; *** P -value losartan treatment vs. no treatment = 0.0005 (B). Values represent mean ± S.E.M., n ≥ 7, unpaired t -test with Welch’s correction used, *** P -value wild-type vs. C7-hypomorph < 0.0001; *** P -value C7-hypomorph + losartan vs. C7-hypomorph = 0.0002; ** P -value wild-type vs. C7-hypomorph + losartan = 0.0016 (D).
Hrp Conjugated Goat Antimouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti p smad3 ps465 467 138d4  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc rabbit anti p smad3 ps465 467 138d4
A–D C7-hypomorphic mice were treated with losartan for 7 weeks, and the forepaws of age-matched untreated, losartan-treated, and wild-type mice were subjected to immunofluorescence staining with antibodies to active thrombospondin 1 (Tsp1, green) (A), active <t>Tgf-β1</t> (green) (B), TGF-β receptor II (Tgfbr2, red) (C), and phospho-Smad2 and 3 (P-Smad2/3, red) (D). The nuclei were counterstained with DAPI (blue). Images were acquired with a 20× objective (scale bar = 100 μm) (A), with a 4× objective (scale bar = 200 μm) (B, D), and with a 40× objective (scale bar = 50 μm) (C). The bar graphs on the right show quantification of the stainings in the left panel of (B) and (D). Paired values were normalized to the staining intensity of untreated C7-hypomorphic paws, which were set to 100%. Values represent mean ± S.E.M., n = 6, paired Student’s t -test, *** P -value wild-type vs. C7-hypomorph receiving no treatment = 0.0004; *** P -value losartan treatment vs. no treatment = 0.0005 (B). Values represent mean ± S.E.M., n ≥ 7, unpaired t -test with Welch’s correction used, *** P -value wild-type vs. C7-hypomorph < 0.0001; *** P -value C7-hypomorph + losartan vs. C7-hypomorph = 0.0002; ** P -value wild-type vs. C7-hypomorph + losartan = 0.0016 (D).
Rabbit Anti P Smad3 Ps465 467 138d4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hrp conjugated goat anti mouse igg  (Jackson Immuno)
96
Jackson Immuno hrp conjugated goat anti mouse igg
A–D C7-hypomorphic mice were treated with losartan for 7 weeks, and the forepaws of age-matched untreated, losartan-treated, and wild-type mice were subjected to immunofluorescence staining with antibodies to active thrombospondin 1 (Tsp1, green) (A), active <t>Tgf-β1</t> (green) (B), TGF-β receptor II (Tgfbr2, red) (C), and phospho-Smad2 and 3 (P-Smad2/3, red) (D). The nuclei were counterstained with DAPI (blue). Images were acquired with a 20× objective (scale bar = 100 μm) (A), with a 4× objective (scale bar = 200 μm) (B, D), and with a 40× objective (scale bar = 50 μm) (C). The bar graphs on the right show quantification of the stainings in the left panel of (B) and (D). Paired values were normalized to the staining intensity of untreated C7-hypomorphic paws, which were set to 100%. Values represent mean ± S.E.M., n = 6, paired Student’s t -test, *** P -value wild-type vs. C7-hypomorph receiving no treatment = 0.0004; *** P -value losartan treatment vs. no treatment = 0.0005 (B). Values represent mean ± S.E.M., n ≥ 7, unpaired t -test with Welch’s correction used, *** P -value wild-type vs. C7-hypomorph < 0.0001; *** P -value C7-hypomorph + losartan vs. C7-hypomorph = 0.0002; ** P -value wild-type vs. C7-hypomorph + losartan = 0.0016 (D).
Hrp Conjugated Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A–D C7-hypomorphic mice were treated with losartan for 7 weeks, and the forepaws of age-matched untreated, losartan-treated, and wild-type mice were subjected to immunofluorescence staining with antibodies to active thrombospondin 1 (Tsp1, green) (A), active Tgf-β1 (green) (B), TGF-β receptor II (Tgfbr2, red) (C), and phospho-Smad2 and 3 (P-Smad2/3, red) (D). The nuclei were counterstained with DAPI (blue). Images were acquired with a 20× objective (scale bar = 100 μm) (A), with a 4× objective (scale bar = 200 μm) (B, D), and with a 40× objective (scale bar = 50 μm) (C). The bar graphs on the right show quantification of the stainings in the left panel of (B) and (D). Paired values were normalized to the staining intensity of untreated C7-hypomorphic paws, which were set to 100%. Values represent mean ± S.E.M., n = 6, paired Student’s t -test, *** P -value wild-type vs. C7-hypomorph receiving no treatment = 0.0004; *** P -value losartan treatment vs. no treatment = 0.0005 (B). Values represent mean ± S.E.M., n ≥ 7, unpaired t -test with Welch’s correction used, *** P -value wild-type vs. C7-hypomorph < 0.0001; *** P -value C7-hypomorph + losartan vs. C7-hypomorph = 0.0002; ** P -value wild-type vs. C7-hypomorph + losartan = 0.0016 (D).

Journal: EMBO Molecular Medicine

Article Title: Losartan ameliorates dystrophic epidermolysis bullosa and uncovers new disease mechanisms

doi: 10.15252/emmm.201505061

Figure Lengend Snippet: A–D C7-hypomorphic mice were treated with losartan for 7 weeks, and the forepaws of age-matched untreated, losartan-treated, and wild-type mice were subjected to immunofluorescence staining with antibodies to active thrombospondin 1 (Tsp1, green) (A), active Tgf-β1 (green) (B), TGF-β receptor II (Tgfbr2, red) (C), and phospho-Smad2 and 3 (P-Smad2/3, red) (D). The nuclei were counterstained with DAPI (blue). Images were acquired with a 20× objective (scale bar = 100 μm) (A), with a 4× objective (scale bar = 200 μm) (B, D), and with a 40× objective (scale bar = 50 μm) (C). The bar graphs on the right show quantification of the stainings in the left panel of (B) and (D). Paired values were normalized to the staining intensity of untreated C7-hypomorphic paws, which were set to 100%. Values represent mean ± S.E.M., n = 6, paired Student’s t -test, *** P -value wild-type vs. C7-hypomorph receiving no treatment = 0.0004; *** P -value losartan treatment vs. no treatment = 0.0005 (B). Values represent mean ± S.E.M., n ≥ 7, unpaired t -test with Welch’s correction used, *** P -value wild-type vs. C7-hypomorph < 0.0001; *** P -value C7-hypomorph + losartan vs. C7-hypomorph = 0.0002; ** P -value wild-type vs. C7-hypomorph + losartan = 0.0016 (D).

Article Snippet: The following antibodies were used: mouse anti-human TSP1 clone A6.1, rabbit anti-P-SMAD2/3 (sc-11769R), rabbit anti-SMAD2/3 (FL-425), and goat anti-LRG1 (P-16) (Santa Cruz Biotechnology, Santa Cruz, CA, USA); rabbit anti-human collagen I (R1038X) (Acris Antibodies, Herford, Germany); rabbit anti-active TGF-β1 (Promega, Madison, WI, USA); mouse anti-human β-actin clone AC-15, mouse anti-human total ERK1/2 clone ERK-NP2, mouse anti-human P-ERK1/2 clone ERK-PT 115, rat anti-mouse tenascin-C clone 578, and mouse anti-human serpin F2 (mAb1470; R&D systems, Minneapolis, MN, USA); rabbit anti-human IL-6 (ab6672), rabbit anti-human fibronectin (ab2413), rabbit anti-human TGFBR2 (ab28382), and rabbit anti-β-tubulin (ab6046) (Abcam, Cambridge, UK); rabbit anti-P-SMAD3 (pS465/467) (138D4) (Cell Signaling, Danvers, MA, USA); rabbit anti-P-SMAD2 (pS423/425) (EP823Y) (Epitomics, Burlingame, CA, USA); rat anti-mouse Cd11b clone M1/70 (BD Biosciences, Heidelberg, Germany); rat anti-mouse C1q clone 7H8 (Hycult Biotech, Uden, the Netherlands); rabbit anti-collagen VII (234192) (Merck Millipore, Billerica, MA, USA); rabbit anti-vitronectin (NBP2-20866) (Novus Biologicals, Littelton, CO, USA); rabbit anti-SERPINF2 (orb101685) (Biorbyt, Cambridge, UK); Cy3-conjugated mouse anti-αSMA clone 2B1 (Sigma-Aldrich, St. Louis, MO, USA); HRP-conjugated goat anti-rabbit IgG and HRP-conjugated goat anti-mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA); and Alexa 488- or 546-conjugated goat anti-rat, anti-rabbit, or anti-mouse IgG (Molecular Probes, Eugene, OR, USA).

Techniques: Immunofluorescence, Staining